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<h1>BBa_K561002</h1>
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<h1>Device</h1>
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<h1>Characterization</h1>
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<p><strong>The expression of RFP under aerobic conditions</strong></p>

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<div id="banner_list"><a href="#" target="_blank"><img
	src="http://2011.igem.org/wiki/images/f/ff/8.1RFPaerobic_fluorescence.png"
	title="" alt="Fluorescenc intensity of RFP under aerobic conditions" /></a>
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	src="http://2011.igem.org/wiki/images/f/f1/8.2RFP_aerobic_lnod.png"
	title="" alt="Growth Rate under aerobic conditions" /></a></div>
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<p>The expression of RFP under aerobic conditions is increasing
during the exponential phase. With the anaerobic promoter, its
expression intensity is very low. The highest expression intensity is
1.12. The PoPS of our device is 0.3065.</p>
<p>&nbsp;</p>
<p><strong>The expression of RFP under anaerobic conditions</strong></p>
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<div id="banner_list1"><a href="#" target="_blank"><img
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	title="" alt="Fluorescenc intensity of RFP under anaerobic conditions" /></a>
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<p>&nbsp;&nbsp;The device of RFP has an anaerobic promoter, which
leads to its high expression under anaerobic conditions, as it shows in
the above graph. It reveals that its highest expression level is 1.12.
The PoPS of our device is 10.45.</p>
<p>&nbsp;</p>
<p><strong>The expression of RFP under micro-aerobic
conditions (2.5%)</strong></p>
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<div id="banner_list2"><a href="#" target="_blank"><img
	src="http://2011.igem.org/wiki/images/0/08/10.1RFP_micro25_fluorescence.png"
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	alt="Fluorescenc intensity of RFP under micro-aerobic conditions (2.5% oxygen)" /></a>
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	src="http://2011.igem.org/wiki/images/4/46/10.2RFP_micro25_od.png"
	title="" alt="Growth Rate under 2.5% oxygen" /></a> <a href="#"
	target="_blank"><img
	src="http://2011.igem.org/wiki/images/4/4d/11.1RFP_micro10_fluorescence.png"
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	title="" alt="Growth Rate under 10% oxygen" /></a></div>
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<p>&nbsp;&nbsp;The anaerobic promoter (PfdhF) has low expression
under micro-aerobic conditions. At 2.5% oxygen and 10% oxygen, its
expression level when reaching the exponential phase is 1.02 and 1.05
respectively. The PoPS of the device at 2.5% oxygen and 10% oxygen are
0.4122 and 0.2127, respectively.</p>
<p>&nbsp;</p>
<p><strong>PoPS of RFP and the expression level of RFP</strong></p>
<p style="text-align: center"><img
	src="http://2011.igem.org/wiki/images/c/c2/12.0PoPS-RFP.png"/></p>
<p>The RFP expression device has an anaerobic promoter, PfdhF. Its
PoPS reaches the highest under anaerobic conditions. Under micro-aerobic
or aerobic conditions,its PoPS is equal to 0 relatively.</p>
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